Elucidating the mechanism of cellular uptake and


25-Jul-2020 19:40

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China.

[email protected] [email protected] and Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and Mc Knight Brain Institute, University of Florida, Gainesville, Florida 32611-7200, USA.

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, 410082, China.

Elucidating the endocytosis and metabolism of nanoparticles in cells could improve the diagnostic sensitivity and therapeutic efficiency.

Although the mechanism of action of biguanides remains imperfectly understood the efficacy of metformin in the treatment of type 2 diabetes is unchallenged.

Metformin was developed based on the natural product galegine.

Initially, metformin was believed to interfere with complex 1 of the respiratory chain leading to a reduced ratio of [ATP]/[ADP.

AMP] activating the enzyme AMP-activated protein kinase (AMPK).

Results demonstrated that both techniques increased particle uptake, with penetratin proving more cell specific.

This study sheds also light on biguanides anti-cancer properties.

A better understanding of biguanides mechanism of action could lead to improved outcomes for millions of diabetics worldwide as well as it could provide a new protein target for the treatment of cancer.

Clathrin- medicated endocytosis appeared to be responsible for uptake as shown PCR and western blot, with Pitstop 2 (known to selectively block clathrin formation) blocking particle uptake.

elucidating the mechanism of cellular uptake and-80

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Interestingly, it was further shown that a magnetic field was able to reverse or overcome the blocking, suggesting an alternative route of uptake.In this work, we explore the cellular uptake mechanism of a biocompatible nanocrystal nanostructure, graphene-isolated-Au-nanocrystals (GIANs), by monitoring the intrinsic Raman and two-photon luminescence signals of GIANs in live cells.